| 流式细胞仪在微型浮游植物生态学中的应用 |
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| 引用本文： | 孙书存,陆健健,张利华. 流式细胞仪在微型浮游植物生态学中的应用[J]. 生态学杂志, 2000, 19(1): 72-78 |
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| 作者姓名： | 孙书存 陆健健 张利华 |
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| 作者单位： | 华东师范大学河口海岸国家重点实验室，上海 200062 |
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| 基金项目： | 河口海岸国家重点实验室与厦门大学海洋生态环境开发室基金资助 |
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| 摘 要： | 微型浮游植物是水域生态系统中的初级生产者 ,是海洋生态系统存在的基础。习惯上浮游植物根据其粒径大小有分为小型浮游植物 (ｍｉｃｒｏ - ｐｈｙｔｏｐｌａｎｋｔｏｎ ,>2 0ｕｍ) ,微型浮游植物 (ｎａｎｏ - ｐｈｙｔｏｐｌａｎｋ ｔｏｎ ,<2 0ｕｍ ,>2ｕｍ )和超微型浮游植物( ｐｉｃｏ - ｐｈｙｔｏｐｌａｎｋｔｏｎ ,<2ｕｍ )三类[13 ] 。本文将后两类统称为微型浮游植物。在早期的多数研究中 ,检测技术和方法落后 :譬如测定水域中的生物量和叶绿素ａ含量常用的拖网方法 ,几乎丢失了所有的微型浮游植物 ;即使是实验室内常用 0 .45ｕｍ…
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| 关 键 词： | 微型浮游植物 生态学 流式细胞仪 |
| 修稿时间： | 1999-02-02 |
Applications of Flow Cytometer in Ecological Studies of Nano- and Pico - phytoplankton |
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| Sun Shucun and Lu Jianjian. Applications of Flow Cytometer in Ecological Studies of Nano- and Pico - phytoplankton[J]. Chinese Journal of Ecology, 2000, 19(1): 72-78 |
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| Authors: | Sun Shucun and Lu Jianjian |
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| Abstract: | Flow cytometer (FCM) can be used to obtain the muti-parameters of the light scattering and fluorescence of individual cell rapidly and conveniently. It has been more and more applied in oceanographic and limnological studies. The work principles of FCM and its applications in the ecological studies of nano- and pico-phytoplankton were introduced and reviewed based on some recent publications abroad and at home in this paper. By means of FCM, sub-populations of phytoplankton can easily be classified, counted and sorted, and then further studies of the adaptive ability and ecological responses of the sub-populations can be implemented. It was concluded that FCM would have a prosperous prospect in the studies of the primary production and the microbial food web in marine ecosystem. |
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| Keywords: | flow cytometer(FCM) muli-parameters measurement nano- and pico- phytoplankton ecology application. |
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