The role of red blood cell adenylyl cyclase activation in changes of erythrocyte membrane microrheological properties |
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Authors: | A V Muravyov V B Koshelev O E Fadukova I A Tikhomirova A A Maimistova S V Bulaeva |
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Institution: | 1.Ushinsky State Pedagogical University,Yaroslavl,Russia;2.Department of Medicine and Physiology,Lomonosov Moscow State University,Moscow,Russia |
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Abstract: | The ability of red blood cells (RBCs) for the reversible change of their shape under passing through capillaries in microcirculation
mainly depends on membrane elasticity of these cells. Phosphorylation of some membrane proteins can result in the changes
of microrheological red blood cell properties. Here we show a significant increase in RBC deformability (RBCD) after incubation
with isoproterenol (10−6 M). Red blood cell aggregation (RBCA) decreased under these conditions only slightly. When forskolin (10 μM), an adenylyl
cyclase (AC) stimulator, was added to the RBC suspension, RBCD increased significantly (p < 0.05). Some more changes of deformability were found after incubation of RBC with stable penetrating analog of cyclic adenosine
phosphate (cAMP), dibutyryl-cAMP, (dB-cAMP, 50 μM) and after phosphodiesterase (PDE) activity inhibition with Vinpocetine,
Rolipram, or IBMX. It was found that Gs-proteins inhibitor Clonidine and specific Gi-protein stimulator Mastaparan 7 increased
both RBCD and RBCA. On the whole, the data clearly show that the RBC aggregation and deformation changes are related with
activation of the different intracellular signaling pathways. We suppose that RBCD increase was mainly associated with activation
of the adenylyl-cyclase-cAMP system. |
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