Multiplex analysis of cytokines in exhaled breath condensate. |
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| Authors: | Ulrich Sack Robert Scheibe Michael W?tzel Stefan Hammerschmidt Hartmut Kuhn Frank Emmrich Gerard Hoheisel Hubert Wirtz Christian Gessner |
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| Affiliation: | Institute of Clinical Immunology and Transfusion Medicine, University of Leipzig, Leipzig, Germany. ulrich.sack@medizin.uni-leipzig.de |
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| Abstract: | BACKGROUND: To improve monitoring of lung diseases, we analyzed cytokines in exhaled breath condensate (EBC). The main challenge in measurement of cytokines in EBC is the low protein content, which requires concentration steps that conflict with the need for excessive fluid required by most commonly used kits. METHODS: Here, a multiplex bead array for the detection of interleukins (IL) -1beta, -6, -8, -10, TNF-alpha, and IL-12p70 was modified and validated for analysis in EBC samples. Furthermore, 33 healthy volunteers and 11 patients with acute lung injury were investigated. RESULTS: In patients with inflammatory lung diseases, cytokine levels for all investigated cytokines were higher in comparison to healthy smokers or healthy volunteers. DISCUSSION: Multiplexed immunoassays in highly sensitive approaches allow for cytokine detection in EBC. We found significant differences between patients and controls for all investigated cytokines. |
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