Potential of microsatellites to distinguish four races of Fusarium oxysporum f. sp. ciceri prevalent in India |
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Authors: | M P Barve M P Haware M N Sainani P K Ranjekar V S Gupta |
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Institution: | (1) Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune, Maharashtra 411 008 INDIA Fax: +91-20-5884032 e-mail: vidya@ems.ncl.res.in; pkr@ems.ncl.res.in, XX;(2) Legumes Pathology Unit, International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Andhra Pradesh 502 324 India, IN |
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Abstract: | Fusarium oxysporum f. sp. ciceri, the causal agent of chickpea wilt, is an important fungal pathogen in India. Thirteen oligonucleotide probes complementary
to microsatellite loci, in combination with 11 restriction enzymes, were used to assess the potential of such markers to study
genetic variability in four Indian races of the pathogen. Hybridisation patterns, which were dependent upon both the restriction
enzyme and oligonucleotide probe used, revealed the presence of different repeat motifs in the F. oxysporum f. sp. ciceri genome. Among the restriction enzymes used, hexa-cutting enzymes were more informative than tetra- and penta-cutting enzymes,
whereas tetranucleotide and trinucleotide repeats yielded better hybridisation patterns than dinucleotide repeats. Dependent
upon the levels of polymorphism detected, we have identified (AGT)5, (ATC)5 and (GATA)4 as the best fingerprinting probes for the F. oxysporum f. sp. ciceri races. The distribution of microsatellite repeats in the genome revealed races 1 and 4 to be closely related at a similarity
index value of 76.6%, as compared to race 2 at a similarity value of 67.3%; race 3 was very distinct at a similarity value
of 26.7%. Our study demonstrates the potential of oligonucleotide probes for fingerprinting and studying variability in the
F. oxysporum f. sp. ciceri races and represents a step towards the identification of potential race diagnostic markers.
Received: 12 March 2000 / Accepted: 14 April 2000 |
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Keywords: | Fusarium oxysporum DNA fingerprinting chickpea |
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