首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Primary cilia in the developing pig testis
Authors:Young Ou  Camila Dores  Jose-Rafael Rodriguez-Sosa  Frans A van der Hoorn  Ina Dobrinski
Institution:1. Cumming School of Medicine, Department of Biochemistry and Molecular Biology, University of Calgary, 406 HMRB, 3330 Hospital Drive NW, AB, T2N 4N1, Calgary, Canada
2. Department of Comparative Biology & Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, 404 HMRB, 3330 Hospital Drive NW, AB, T2N 4N1, Calgary, Canada
Abstract:In vertebrates, a variety of cell types generate a primary cilium. Cilia are implicated in determination and differentiation of a wide variety of organs and during embryonic development. However, there is little information on the presence or function of primary cilia in the mammalian testis. Therefore, the objective of this study was to characterize expression of primary cilia in the developing pig testis. Testicular tissue from pigs at 2–10 weeks of age was analyzed for primary cilia by immunocytochemistry. Expression of primary cilia was also analyzed in testicular tissue formed de novo from a single cell suspension ectopically grafted into a mouse host. Functionality of primary cilia was monitored based on cilia elongation after exposure to lithium. Analysis showed that the primary cilium is present in testis cords as well as in the interstitium of the developing pig testis. Germ cells did not express primary cilia. However, we identified Sertoli cells as one of the somatic cell types that produce a primary cilium within the developing testis. Primary cilium expression was reduced from the second to the third week of pig testis development in situ and during de novo morphogenesis of testis tissue from a single cell suspension after xenotransplantation. In vitro, primary cilia were elongated in response to lithium treatment. These results indicate that primary cilia on Sertoli cells may function during testicular development. De novo morphogenesis of testis tissue from single cell suspensions may provide an accessible platform to study and manipulate expression and function of primary cilia.
Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号