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Expression of Group B Protective Surface Protein (BPS) by Invasive and Colonizing Isolates of Group B Streptococci |
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| Authors: | Aurea E. Flores G. S. Chhatwal Sharon L. Hillier Carol J. Baker Patricia Ferrieri |
| Affiliation: | 1. Department of Pediatrics and Laboratory Medicine and Pathology, University of Minnesota Medical School, MMC 134, 420 Delaware St. SE, Minneapolis, MN, 55455, USA 2. Department of Microbial Pathogenesis and Vaccine Research, GBF-German Research Centre for Biotechnology, 38124, Brunswick, Germany 3. Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Women’s Research Institute and University of Pittsburgh School of Medicine, Pittsburgh, PA, USA 4. Departments of Pediatrics, Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA |
| Abstract: | Group B protective surface protein (BPS) is expressed on the cell surface of some group B streptococcal (GBS) (Streptococcus agalactiae) strains and adds to the identification by capsular polysaccharide (CPS), and c or R proteins. We investigated the prevalence of BPS among GBS clinical isolates (303 invasive, 4122 colonizing) collected over 11 years in four American cities. Hot HCl cell extracts were tested by immunoprecipitation in agarose with rabbit antisera to BPS; the alpha (α) and beta (β) components of c protein; R1, R3, and R4 species of R protein; and CPS serotypes Ia–VIII. BPS was found in 155 isolates (seven invasive, 148 colonizing). Of these, 87 were Ia, 37 II, 20 V; none were III. BPS was expressed usually with another protein: a species of R by 87 or a component of c by 39. The predominant CPS/protein profiles with BPS were Ia/R1,BPS and II/c(α + β),BPS. Thus, along with CPS serotype and other surface proteins, BPS can be a valuable marker for precise strain characterization of unique GBS clinical isolates with complex surface protein profiles. |
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