Immunohistochemical demonstration of alpha-1-antitrypsin in the islet cells of human pancreas |
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Authors: | Dr. M. B. Ray V. J. Desmet |
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Affiliation: | (1) Department of Embryology and Comparative Histology, Rijksuniversiteit, Gent, Belgium;(2) Laboratorium voor Embryologie en Vergelijkende Histologie, Rijksuniversiteit, Godshuizenlaan, 4, B-9000 Gent, Belgium |
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Abstract: | Summary Using the unlabeled antibody peroxidase-antiperoxidase complex (PAP) technique at the light microscopic level, it has been shown that, in the dipnoan preoptico-hypophysial neurosecretory system, vasotocin and mesotocin are synthesized in separate neurons. In the preoptic nuclei, the perikarya of these two types of neurosecretory neurons are not located preferentially. The two types of neurosecretory perikarya give rise to separate vasotocinergic and mesotocinergic axons, respectively. The dipnoan median eminence and neural lobe contain separate vasotocinergic and mesotocinergic nerve fibres, the general distribution of which is described. In the pars distalis and the pars intermedia of the hypophysis, neurohypophysial hormone-containing nerve fibres have not been found.This investigation was supported by a grant from the Belgian Nationaal Fonds voor Geneeskundig Wetenschappelijk OnderzoekThe authors are greatly indebted to Prof. Dr. Hyder, Department of Zoology, University of Nairobi, Kenya for kindly supplying us with the fixed material used in this study |
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Keywords: | Hypothalamus Dipnoi (Protopterus) Neurohypophysial hormones Immunocytochemistry |
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