| 中国鄂温克、鄂伦春、达斡尔族永生细胞系的建立与保存 |
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| 引用本文： | 薛雅丽,王琦,史忠诚,刘岸,张钰,黄小义,黄承滨,陈白滨,杨焕杰,傅松滨,李璞.中国鄂温克、鄂伦春、达斡尔族永生细胞系的建立与保存[J].遗传,2000,22(3):157-158. |
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| 作者姓名： | 薛雅丽 王琦 史忠诚 刘岸 张钰 黄小义 黄承滨 陈白滨 杨焕杰 傅松滨 李璞 |
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| 作者单位： | 哈尔滨医科大学医学遗传学研究室,黑龙江,哈尔滨,150086 |
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| 基金项目： | 国家自然科学基金资助项目，黑龙江省科学技术攻关项目 |
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| 摘 要： | 本采用ＥＢＶ（Ｅｐｓｔｅｉｎ－Ｂａｒｒ Ｖｉｒｕｓ）上清液转化Ｂ淋巴细胞，并加入环胞霉素Ａ（Ｃｙｃｌｏｓｐｏｒｉｎｅ Ａ）抑制Ｔ淋巴细胞，成功地对中国东北地区鄂温克族、鄂伦春族及达斡尔族的部分个体建立了永生细胞系，其中鄂温克族４９株，鄂伦春族４０株，达斡尔族５１例，总计１４０株。永久保存我国特有民族的基因组，为分析其遗传学差异奠定了基础。
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| 关 键 词： | 永生细胞系 EBV 少数民族 鄂温克族 鄂伦春族 |
| 修稿时间： | 1999－08－05 |
Establishment and Preservation of Immortal Lymphoblastoid Cell Lines of the Ewenki,the Oroqen and the Daur Ethnic Groups in China |
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| XUE Ya-li,WANG Qi,SHI Zhong-cheng,LIU An,ZHANG Yu,HUANG Xiao-yi,HUANG Cheng-bin,CHEN Bai-bin,YANG Huan-jie,FU Song-bin,LI Pu.Establishment and Preservation of Immortal Lymphoblastoid Cell Lines of the Ewenki,the Oroqen and the Daur Ethnic Groups in China[J].Hereditas,2000,22(3):157-158. |
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| Authors: | XUE Ya-li WANG Qi SHI Zhong-cheng LIU An ZHANG Yu HUANG Xiao-yi HUANG Cheng-bin CHEN Bai-bin YANG Huan-jie FU Song-bin LI Pu |
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| Abstract: | The immortal lymphoblastoid cell lines were established by EBV transformation of B cells and addition of cyclosporin A to inhabit the activity of T cells.In the present study ,140 immortal cell lines of the Ewenki,the Oroqen and the Daur ethnic groups in the Northeast China were established .This is an important part of the research of human genome diversity for the exploration of the origin and progression of different ethnic groups ,and also provide enough research materials for further studies. |
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| Keywords: | immortal lymphoblastoid cell line EBV ethnic groups |
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