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DNA methylation in imprinted genes IGF2 and GNASXL is associated with prenatal maternal stress
Authors:E B Vangeel  B Izzi  T Hompes  K Vansteelandt  D Lambrechts  K Freson  S Claes
Institution:1. Genetic Research about Stress and Psychiatry (GRASP), Department of Neurosciences, Leuven, Belgium;2. Center for Molecular and Vascular Biology (CMVB), Department of Cardiovascular Sciences, University of Leuven, Leuven, Belgium;3. University Psychiatric Center, University of Leuven, Leuven, Belgium;4. Laboratory of Translational Genetics, Department of Oncology, University of Leuven, Leuven, Belgium;5. Vesalius Research Center (VRC), VIB, Leuven, Belgium
Abstract:Epigenetic regulation of imprinted genes during embryonic development is influenced by the prenatal environment. Our aim was to examine the effect of maternal emotional stress and cortisol levels during pregnancy on methylation of imprinted genes, insulin‐like growth factor 2 (IGF2) and guanine nucleotide‐binding protein, alpha stimulating extra‐large (GNASXL), using umbilical cord blood DNA. Maternal depressed mood (Edinburgh Depression Scale; EDS), pregnancy‐related anxiety questionnaire (PRAQ) and cortisol day profiles were assessed throughout pregnancy. At birth, a cord blood sample (n = 80) was taken to study DNA methylation of IGF2 DMR0 (differentially methylated region), IGF2 anti‐sense (IGF2AS) and GNASXL using Sequenom Epi TYPER. Linear mixed models were used to examine the relationship between DNA methylation and maternal stress, while correcting for confounders. We also studied the association of DNA methylation with the child ponderal index at birth. We found a cytosine–guanine dinucleotide (CpG)‐specific association of PRAQ subscales with IGF2 DMR0 (CpG5, P < 0.0001) and GNASXL (CpG11, P = 0.0003), while IGF2AS was associated with maternal EDS scores (CpG33, P = 0.0003) and cortisol levels (CpG33, P = 0.0006; CpG37‐38, P = 0.0005). However, there was no association of methylation with ponderal index at birth. In conclusion, maternal stress during pregnancy, as defined by cortisol measurements, EDS and PRAQ scores, is associated with DNA methylation of imprinted genes IGF2 and GNASXL. Our results provide further evidence that prenatal adversity can influence imprinted gene methylation, although future studies are needed to unravel the exact mechanisms.
Keywords:Anxiety  cortisol  depression  development  DNA methylation  epigenetics  GNAS  IGF2  imprinting  prenatal stress
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