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Functional diversity of human protein kinase splice variants marks significant expansion of human kinome
Authors:Krishanpal Anamika  Nicolas Garnier  Narayanaswamy Srinivasan
Affiliation:1. Department of Biochemistry, Li Ka Shing Faculty of Medicine, University of Hong Kong, Pok Fu Lam, Hong Kong SAR, PR China
2. Oral Biosciences, Faculty of Dentistry, University of Hong Kong, Pok Fu Lam, Hong Kong SAR, PR China
3. Genetics of Bacterial Genomes, CNRS URA2171, Institut Pasteur, 28, rue du Docteur Roux, Paris Cedex 15, 75724, France
Abstract:

Background

The low pH environment of the human stomach is lethal for most microorganisms; but not Escherichia coli, which can tolerate extreme acid stress. Acid resistance in E. coli is hierarchically controlled by numerous regulators among which are small noncoding RNAs (sncRNA).

Results

In this study, we individually deleted seventy-nine sncRNA genes from the E. coli K12-MG1655 chromosome, and established a single-sncRNA gene knockout library. By systematically screening the sncRNA mutant library, we show that the sncRNA GcvB is a novel regulator of acid resistance in E. coli. We demonstrate that GcvB enhances the ability of E. coli to survive low pH by upregulating the levels of the alternate sigma factor RpoS.

Conclusion

GcvB positively regulates acid resistance by affecting RpoS expression. These data advance our understanding of the sncRNA regulatory network involved in modulating acid resistance in E. coli.
Keywords:
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