狂犬病毒CTN—1株在Vero细胞上的适应传代研究

本文报导了用我国狂犬病毒固定毒人二倍体细胞适应株（ＣＴＮ－１）进行Ｖｅｒｏ细胞适应传代研究。通过连续传代培养，滴度可达８．０１ｏｇＬＤ５０／ｍｌ，达到了ＷＨＯ规定的不需浓缩的标准。病毒用０．０１ＭＯＩ感染细胞其产量与１Ｍｏｌ感染量相仿。病毒增殖高峰在４－５天，维持达１５天无明显下降，且可连续收获４－５次。因此，该毒种符合ＷＨＯ提出的疫苗生产毒种要求，可用于狂犬病疫苗生产。

Studies of Adaptation and Passage on Rabies Virus (CTN-1)Strain in Vero Cell Cultures

The purified Vero cell rabies vaccine is one of the most successfuly developed new rabies vaccine. It's potency, stability and human trials have been shown satisfactory. We have now adapted a rabies virus strain in Vero cells. The results showed as follows. The CTN-1 (37) strain of rabies virus were adapted in Vero cell by consecutive passage. The titer of virus on first passage was 7. 0 Log LD_(50)/ml after adaptation it reached to 8.0 Log LD50/ml in the 20th passage, The virus yeild with 0. 01 M. O. I. inoculation in Vero cell was similar with 1. 0 M. O. I. The peak of virus titer was found in day 4-5. Several harvests of virus fluid with similar high titer could be obtained beginning from day 5 to day 15. It is considered that this adapted strain could be used as vius seed for Vero cell rabies vaccine.