Detection byin-situ fluorescence of short,single-copy sequences of chromosomal DNA |
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Authors: | Tong Zhu Liang Shi Paul Keim |
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Institution: | (1) Department of Biological Sciences, Northern Arizona University, 86011-5640 Flagstaff, Arizona, USA |
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Abstract: | Short single-copy probes have been widely used in plant molecular biology. However, they have rarely been effective in plant
research usingin situ hybridization techniques, possibly due to limitations imposed by the cell wall. We recently developed two fluorescencein situ hybridization protocols for the single-copy sequence detection in soybean. By enzymatically removing the cell wall, single-copy
sequences as short as 1 kb were detected by probes using standard fluorescencein situ hybridization or PCR-primedin situ hybridization (PCR-PRINS). Such technology is useful for genome analysis, in plant molecular, cellular, and biotechnological
research. |
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Keywords: | Fluorescencein situ hybridization PCR-primedin situ hybridization (PCR-PRINS) RFLP loci soybean (Glycine max) |
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