Determination of nandrolone and metabolites in urine samples from sedentary persons and sportsmen |
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Authors: | A. M. Gal n Martí n, J. I. Maynar Mari o, M. P. Garcí a de Tiedra, J. J. Rivero Marab , M. J. Caballero Loscos,M. Maynar Mari o |
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Affiliation: | A. M. Galán Martín, J. I. Maynar Mariño, M. P. García de Tiedra, J. J. Rivero Marabé, M. J. Caballero Loscos,M. Maynar Mariño |
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Abstract: | Metabolites of nandrolone were determined in the urine of several sportsmen, sedentary and post-menopausal women by capillary gas chromatography–mass spectrometry quadrupole (GC–MS) and capillary gas chromatography mass–mass spectrometry ion trap (GC–MS–MS) methods. The method employed was GC–EI-MS with 17α-methyltestosterone as internal standard with ethyl ether extraction prior to selected ion monitoring of the bis(trimethylsilyl) ethers at ion masses m/z 405 and 420 for the nandrolone metabolites, and 418 and 403 for nandrolone derivative. Recovery for nandrolone, 19-norandrosterone (19-NA) and 19-noretiocholanolone (19-NE) was 97.20, 94.17 and 95.54%, respectively. Detection limits for nandrolone, 19-NA and 19-NE were 0.03, 0.01 and 0.06 ng/ml. Metabolites of nandrolone (19-NA and 19-NE) were found in 12.5% (n=40) of sportsmen and 40% (n=10) of post-menopausal women. |
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Keywords: | Nandrolone 19-Norandrosterone 19-Noretiocholanolone |
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