Degradation of ethylbenzene by free and immobilized <Emphasis Type="Italic">Pseudomonas</Emphasis><Emphasis Type="Italic">fluorescens-</Emphasis>CS2 |
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Authors: | Suneetha Parameswarappa Chandrakant Karigar Manjunath Nagenahalli |
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Institution: | (1) Bioremediation Laboratory, Biochemistry Division, Department of Chemistry, Central College Campus, Bangalore University, Bangalore, Karnataka, 560001, India |
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Abstract: | Pseudomonas
fluorescens-CS2 metabolized ethylbenzene as the sole source of carbon and energy. The involvement of catechol as the hydroxylated intermediate
during the biodegradation of ethylbenzene was established by TLC, HPLC and enzyme analysis. The specific activity of Catechol
2,3-dioxygenase in the cell free extracts of P.
fluorescens-CS2 was determined to be 0.428 μmoles min−1 mg−1 protein. An aqueous-organic, Two-Phase Batch Culture System (TPBCS) was developed to overcome inhibition due to higher substrate
concentrations. In TPBCS, P. fluorescens-CS2 demonstrated ethylbenzene utilization up to 50 mM without substrate inhibition on inclusion of n-decanol as the second phase. The rate of ethylbenzene metabolism in TPBCS was found enhance by fivefold in comparison with
single phase system. Alternatively the alginate, agar and polyacrylamide matrix immobilized P. fluorescens-CS2 cells efficiently degraded ethylebenzene with enhanced efficiency compared to free cell cultures in single and two-phase
systems. The cells entrapped in ployacrylamide and alginate were found to be stable and degradation efficient for a period
of 42 days where as agar-entrapped P. fluorescens was stable and efficient a period of 36 days. This demonstrates that alginate and polyacrylamide matrices are more promising
as compared to agar for cell immobilization. |
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Keywords: | Ethylbenzene Pseudomonas fluorescens Biodegradation TPBCS catechol Immobilization |
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