The glucose repression and RAS-cAMP signal transduction pathways of Saccharomyces cerevisiae each affect RNA processing and the synthesis of a reporter protein |
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Authors: | Kuei-Shu Tung and Anita K Hopper |
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Institution: | (1) Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, 500 University Drive, 17033 Hershey, PA, USA;(2) Present address: Department of Biology, Yale University, P.O. Box 6666, 06511-8112 New Haven, CT, USA |
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Abstract: | Previously we reported that mutations in the Saccharomyces cerevisiae REG1 gene encoding a negative regulator of glucose-repressible genes, suppress the RNA processing defects and temperature-sensitive growth of rna1-1 and prp cells. This result and the fact that growth on non-glucose carbon sources also suppresses rna1-1 led us to propose that RNA processing and export of RNA from the nucleus are responsive to carbon source regulation. To understand how carbon source affects these processes, we used p70, an antigen regulated by REGI and by glucose availability, as a reporter. We found that the response of p70 to glucose availability is mediated by both the SNFI-SSN6-dependent glucose repression and the RAS-cAMP pathways. These results led us to test whether the RAS-cAMP pathway interacts with RNA1. We found that suppression of rnal-1 appears to be mediated, at least in part, by the RAS-cAMP pathway. |
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Keywords: | Saccharomyces cerevisiae RNA processing Signal transduction Glucose repression RNA1 |
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