Polypeptides from maize seedlings with protein kinase functions |
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Authors: | Grazyna Musźyna Dobrowolska Elzbieta Ber |
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Affiliation: | Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 36 Rakowiecka St., 02-532 Warszawa, Poland |
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Abstract: | Low molecular weight protein kinase from maize seedlings was isolated. The molecular weight of the enzyme was below 10 000. The enzyme preferentially utilized ATP and casein as donor and acceptor of phosphorus, respectively. Casein kinase was a cyclic nucleotide-independent, heparin-sensitive enzyme. The low molecular weight casein kinase was resolved into basic, neutral and slightly acidic peaks of activity by chromatofocussing. |
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