Characterization of oligosaccharide units of p-N-collagen type III from dermatosparactice bovine skin |
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| Authors: | Hiroshi Shinkai Charles M. Lapiere |
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| Affiliation: | 1. Department of Dermatology, Medical College of Oita, Oita, Japan;2. Department of Dermatology, University of Liège, Bld. de la Constitution, 66, B-4020 Liège, Belgium |
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| Abstract: | p-N-collagen type III was extracted from dermatosparactic and normal fetal bovine skin and purified by ion-exchange chromatography using DEAE- and CM-cellulose. Asparagine-linked sugar chains were fractionated by high voltage paper chromatography from the products obtained after hydrazinolysis and reduction with NaB3H4. These oligosaccharides composed of neutral and acidic components are mannose-containing oligosugars of the complex type. Their abundance is much higher in dermatosparactic p-N-collagen type III. |
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| Keywords: | Oligosaccharide srtructure, Collagen type III (Bovine skin) |
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