Calmodulin-stimulated cyclic nucleotide phosphodiesterase from Neurosproa crassa |
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| Authors: | Ruben Ortega Perez Diederik Van Tuinen Dieter Marmé Gilbert Turian |
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| Affiliation: | 1. Laboratoire de Microbiologies Générale, Département de Biologie Végétale, Université de Genève, CH-1211 Geneva 4 Switzerland;2. Institut für Biologie III, University of Freiburgh, D-7800 Freiburg i.Br. F.R.G. |
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| Abstract: | Cyclic nucleotide phosphodiesterase has been partially purified by calmodulin-Sepharose affinity chromatography from a soluble extract of Neurospora crassa. The phosphodiesterase activity remained bound to the affinity column even in the presence of 6 M urea and could only be eluted by calcium chelation. The enzyme exhibits cAMP and cGMP phosphodiesterase activities. Both activities can be enhanced by calmodulin in a Ca2+-dependent manner. Stimulation of cyclic nucleotide phosphodiesterase by calmodulin can be inhibited by calmodulin antagonists such as pimozide, trifluoperazine and chlorpromazine. |
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| Keywords: | Calmodulin stimulation Phosphodiesterase Cyclic nucleotide (N. crassa) |
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