Different biosynthetic pathways of the pyrimidine moiety of thiamin in procaryotes and eucaryotes |
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Authors: | Kazuko Yamaada Mayumi Morisaki Hiroshi Kumaoka |
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Institution: | Faculty of Pharmaceutical Sciences, Mukogawa Women''s University, Nishinomiya, Hyogo 663, Japan |
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Abstract: | 14C]Formate is incorporated into the C-2 of the pyrimidine moiety of thiamin by Escherichia coli and Salmonella typhimurium. In Saccharomyces cerevisiae, it is incorporated into C-4. Radioactive carbons of 1-14C]glycine and 2-14C]glycine are incorporated by S. typhimurium into the C-4 and C-6 of the pyrimidine, respectively, but not by S. cerevisiae. These facts suggest that procaryotes and eucaryotes have different biosynthetic pathways for pyrimidine. In this study, the procaryotes tested incorporated 14C]formate into the C-2 and the eucaryotes incorporated it into the C-4 of the pyrimidine. |
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Keywords: | Elastase-like activity Serine peptidase Membrane enzyme (Porcine aorta) |
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