Forssmann antigenicity of chemically synthesized globopentaose |
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| Authors: | Shigeko Kijimoto-Ochiai Akira Makita Almuth Bünsch Hans Paulsen |
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| Institution: | 1. Biochemistry Laboratory, Cancer Institute, Hokkoido University of Mecidine, Sapporo 060 Japan;2. Institute Für Organische Chemie und Biochemie der Universität Martin-Luther-King-Platz 6, D-2000 Hamburg 13 F.R.G. |
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| Abstract: | The Forssman antigenicity of a chemically synthesized globopentaose was studied. Globobentaose at 40 ng showed strong inhibitory activity for the formation of a precipitin line between globopentaosylceramide (Forssman glycolipid) and anit-Forssmann rabbit antiserum, while much more pentasaccharide (7 and 100 μg, respectively) was required to inhibit a 50% quantitative precipitin reaction and a hemolysis reaction. An immune complex of the 3H-labeled globopentaose with anti-Forssman antibody was hardly formed. Thus, the chemically synthesized globopentaose possesses the same antigenic specificity as globopentaosylceramide but it is difficult to achieve a stable complex with Forssman antibody. |
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| Keywords: | Forssman antigen Globopentaose |
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