Inducing and isolation of antibacterial peptides from oriental fruit fly, Bactrocera dorsalis Hendel |
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| Authors: | XIANG-LI DANG JIN-HUAN TIAN HUI-YU YI WEN-XIAN WANG MIN ZHENG YI-FENG LI YANG CAO SHUO-YANG WEN |
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| Affiliation: | Department of Entomology, South China Agricultural University;, Department of Material Science and Engineering, Jinan University;, Department of Sericulture Science, College of Animal Science, South China Agricultural University, Guangzhou, China |
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| Abstract: | One antibacterial activity fraction from an immunized dipteran insect, Bactrocera dorsalis, was isolated and purified by prepurification, ion‐exchange chromatography, gel filtration chromatography and reverse‐phase high performance liquid chromatography (HPLC). The final purified fraction was checked on the Smart system HPLC and was judged as a pure fraction. The results of physical and biological analysis revealed that this fraction is heat stable and showed strong activities against Gram‐positive bacterial growth. It possesses antibicrobial peptide properties and is worth further investigation. |
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| Keywords: | antibacterial peptides Bactrocera dorsalis gel filtration chromatography ion-exchange chromatography reverse-phase HPLC |
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