Polymorphism of the bm86 Gene in South American Strains of the Cattle Tick Boophilus microplus |
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Authors: | Sidimar Sossai Ana P Peconick Policarpo A Sales-Junior Francismar C Marcelino Marlene I Vargas Elisangela S Neves Joaquín H Patarroyo |
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Institution: | 1. Laboratory of Biology and Control of Haematozoa and Vectors, BIOAGRO/Veterinary Department, Federal University of Vi?osa, 36570-000, Vi?osa, MG, Brazil
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Abstract: | Thirty Boophilus microplus strains from various geographic regions of Brazil, Argentina, Uruguay, Venezuela and Colombia were analyzed for the bm86 and bm95 gene. A fragment of cDNA of 794 base pairs of the parasite larvae, included between nucleotides 278–1071s, was amplified
and cloned on the pGEM-T vector. Two random clones were sequenced for each population and the nucleotides 278–1071 and predicted
amino acid sequences compared with the bm86 and bm95 genes. Variations from 1.76 to 3.65% were detected in the nucleotides sequence when compared with the homologous sequence
of the bm86 gene and a 3.4–6.08% in the homologous amino acid sequence of the Bm86 protein. When the sequences obtained were compared
with the bm95 gene, variations from 0.50 to 3.15% were detected. Variations from 1.14 to 4.56% were detected for the Bm95 protein homologous
sequences in the deduced amino acid sequence. Only five of the 30 strains analyzed presented two different types of alleles
expressed and the two alleles of the Alegre population and allele 1 of the Betim population were the most divergent of all
those analyzed. |
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Keywords: | Boophilus microplus bm86 and bm95 genes Bm86 and Bm95 proteins Genetic variability |
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