Manipulation by culture mixing and elicitation of paclitaxel and baccatin III production in Taxus baccata suspension cultures |
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Authors: | M Bonfill O Expsito E Moyano R M Cusid J Palazn M T Piol |
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Institution: | (1) Sección de Fisiología Vegetal, Facultad de Farmacia, Universidad de Barcelona, Avda. Diagnonal 643, E-08028 Barcelona, Spain;(2) Department de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Avda. Dr Aiguader 80, E-08003 Barcelona, Spain |
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Abstract: | Summary Experiments were carried out with Taxus baccata cell lines showing different paclitaxel-producing capacities (between 1.74 and 19.91 mgl−1) when growing in a selected product-formation medium that specifically stimulated the production of taxane to the detriment
of cell growth. Through mixing low-, medial- and high-producing lines, it could be observed that paclitaxel productivity in
the resulting mixed lines was clearly higher than the mean productivity of the individual lines before mixing. This suggests
that culture components generated by high-producing individual lines within the population might induce paclitaxel production.
Although the accumulation of paclitaxel and baccatin III was higher when 100 μM methyl jasmonate was added to the subcultures of the mixed lines, the results indicate that exogenously applied methyl jasmonate
was not the first factor to stimulate taxane production. The possible effects of methyl jasmonate elicitation and paclitaxel
accumulation on cell viability are also considered. |
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Keywords: | cell line mixing cell viability methyl jasmonate taxanes Taxus species |
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