A mathematical analysis of enzyme stabilization by a series-type mechanism: Influence of chemical modifiers

A series-type enzyme deactivation model is utilized to theoretically analyze and to quantify the effect of chemical modifier concentration on the eventual level of enzyme activity stabilization, alpha(2). An increase in the concentration of phosphate ion and NADP increases alpha(2) for the enzymes studied. One example of each enzyme deactivation is given wherein the introduction of chemical modifiers changes the deactivation mechanism from a single-step to a series-type mechanism, and from a series-type to a single-step mechanism. Simple empirical equations are proposed to quantify the effect of chemical modifier concentration on alpha(2).