Subcellular localization of calcium in sporangiophores of Phycomyces blackesleeanus |
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| Authors: | Marisela Morales José Ruiz-Herrera |
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| Institution: | (1) Departamento de Génetica y Biología Molecular, Centro de Investigación y Estudios Avanzados del IPN, México, D.F.;(2) Instituto de Investigación en Biología Experimental, Facultad de Química, Universidad de Guanajuato, Guanajuato, México;(3) Present address: University of Colorado, Boulder, Campus Box 345, 80309-0345 Boulder, CO, USA |
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| Abstract: | The in situ localization of Ca2+ in stage I sporangiophores of the fungus Phycomyces blakesleeanus was achieved with the potassium pyroantimonate technique. Precipitates of calcium-antimonate were present in mitochondria, vacuoles, endoplasmic reticulum and adjacent cytoplasm,  Golgi-like bodies, and nuclei but not cell walls. Material treated with the calcium chelator EGTA lacked these precipitates. The preferential localization of Ca2+ in mitochondria, endoplasmic reticulum and vacuoles suggests that these organelles modulate the level of this cation in sporangiophores of P. blakesleeanus.Abbreviations EGTA
ethyleneglycol-bis-( -aminoethyl ether) N,N, tetraacetic acid |
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| Keywords: | Calcium Phycomyces blakesleeanus Sporangiophore |
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