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mTRAQ‐based quantification of potential endometrial carcinoma biomarkers from archived formalin‐fixed paraffin‐embedded tissues |
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| Authors: | Leroi V DeSouza Olga Krakovska Marlene M Darfler David B Krizman Alexander D Romaschin Terence J Colgan K W Michael Siu |
| Institution: | 1. Department of Chemistry and Centre for Research in Mass Spectrometry, York University, Toronto, ON, Canada;2. Expression Pathology Inc., Rockville, MD, USA;3. Division of Clinical Biochemistry, St. Michael's Hospital, Toronto, ON, Canada;4. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada;5. Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, ON, Canada |
| Abstract: | Formalin‐fixed paraffin‐embedded (FFPE) tissues are the primary and preferred medium for archiving patients' samples. Here we demonstrate relative quantifications of protein biomarkers in extracts of laser microdissected epithelial cells from FFPE endometrial carcinoma tissues versus those from normal proliferative endometria by means of targeted proteomic analyses using LC–multiple reaction monitoring (MRM) MS with MRM Tags for Relative and Absolute Quantitation (mTRAQ) labeling. Comparable results of differential expressions for pyruvate kinase isoform M2 (PK‐M2) and polymeric Ig receptor were observed between analyses on laser microdissected epithelial cells from FFPE tissues and corresponding homogenates from frozen tissues of the same individuals that had previously been analyzed and reported. We also identified PK‐M2 in the normal proliferative phase of the endometrium. Other biomarkers in addition to PK‐M2 and polymeric Ig receptor were also observed but not consistently and/or were at levels below the threshold for quantification. |
| Keywords: | Biomarker Cancer Isotope‐coded affinity tags LC Quantitative analysis Technology |