Structure‐activity relationship of indolicidin,a Trp‐rich antibacterial peptide |
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| Authors: | Setsuko Ando Keitarou Mitsuyasu Yoshitake Soeda Mariko Hidaka Yuki Ito Kouki Matsubara Mitsuno Shindo Yoshiki Uchida Haruhiko Aoyagi |
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| Affiliation: | 1. Department of Chemistry, Faculty of Science, Fukuoka University, Jonan‐ku, Fukuoka 814‐0180, Japan;2. Department of Food Science and Nutrition, Osaka Shoin Woman's University, Higashi‐Osaka 577‐8550, Japan;3. Kyushu Nutrition Welfare University, Kokurakita‐ku, Kitakyushu 803‐8511, Japan |
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| Abstract: | A series of Trp and Arg analogs of antibacterial indolicidin (Ind) was synthesized and the antimicrobial and hemolytic activities were investigated. [L9]Ind, [L11]Ind, [K8,L9]Ind and [K6, 8,L9]Ind showed desirable characteristics, exhibiting negligible hemolytic activity while keeping strong antibacterial activity. The results indicated that the Trp residue at position 11 essentially contributes to both activities and one can not be exchanged for the other, whereas the Trp residues at positions 4 and 9 play important roles in antimicrobial and hemolytic activities, respectively. The Trp residues at positions 6 and 8 play no important roles in biological activities. We then found that the retro analog of Ind showed higher antibacterial activity than Ind against both Gram‐positive and Gram‐negative bacteria but remarkably lower hemolytic activity than that of Ind. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd. |
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| Keywords: | indolicidin antimicrobial peptide retro peptide antibiotic activity hemolytic activity |
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