Proteome analysis of brain in murine experimental autoimmune encephalomyelitis |
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| Authors: | Abolhassan Shahzadeh Fazeli Davood Nasrabadi Mohammad Hossein Sanati Alireza Pouya Saleh M Ibrahim Hossein Baharvand Ghasem Hosseini Salekdeh |
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| Institution: | 1. Department of Molecular Systems Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran;2. Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;3. Department of Medical Genetic, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran;4. Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran;5. Department of Dermatology and Center of Inflammation Medicine, University of Luebeck, Luebeck, Germany;6. Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran;7. Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran |
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| Abstract: | Multiple sclerosis is considered a prototype inflammatory autoimmune disorder of the CNS. Experimental autoimmune encephalomyelitis (EAE) induced by myelin oligodendrocyte glycoprotein is one of the best‐characterized animal models of multiple sclerosis. Comprehensive understanding of gene expression in EAE can help identify genes that are important in drug response and pathogenesis. We applied a 2‐DE‐based proteomics approach to analyze the protein expression pattern of the brain in healthy and EAE samples. Of more than 1000 protein spots we analyzed, 70 showed reproducible and significant changes in EAE compared to controls. Of these, 42 protein spots could be identified using MALDI TOF‐TOF‐MS. They included mitochondrial and structural proteins as well as proteins involved in ionic and neurotransmitter release, blood barriers, apoptosis, and signal transduction. The possible role of these proteins in the responses of mice to animal models of multiple sclerosis is discussed. |
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| Keywords: | 2‐DE Cell Biology Expression profiling Multiple sclerosis |
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