Two‐dimensional proteome reference map for the radiation‐resistant bacterium Deinococcus geothermalis |
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| Authors: | Christina Liedert Jörg Bernhardt Dirk Albrecht Birgit Voigt Michael Hecker Mirja Salkinoja‐Salonen Peter Neubauer |
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| Institution: | 1. Bioprocess Engineering Laboratory, Department of Process and Environmental Engineering and Biocenter Oulu, University of Oulu, Linnanmaa, Oulu, Finland;2. Institute for Microbiology, Ernst‐Moritz‐Arndt‐Universit?t Greifswald, Greifswald, Germany;3. Division of Microbiology, Department of Applied Chemistry and Microbiology and the CoE “Photobiomics” of the Academy of Finland, University of Helsinki, Finland |
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| Abstract: | 2‐DE reference maps for Deinococcus geothermalis cytosolic and cell envelope proteomes were constructed. In total, 403 spots were identified as 299 different proteins. Unique in the proteomes were four subunits of V‐type ATPase and Deinococcus specific proteins constituting one‐fourth of cell envelope proteome. The cytoplasmic proteome included enzymes of the central carbon metabolism, chaperones, enzymes of protein and DNA repair, and oxidative stress. A total of 34 abundant proteins with unknown function may relate to the extreme stress tolerance of D. geothermalis. |
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| Keywords: | 2‐DE Deinococcus geothermalis Microbiology Proteome reference map |
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