‘Sulfo‐click’ for ligation as well as for site‐specific conjugation with peptides,fluorophores, and metal chelators |
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| Authors: | Dirk T. S. Rijkers Remco Merkx Cheng‐Bin Yim Arwin J. Brouwer Rob M. J. Liskamp |
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| Affiliation: | Division of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Department of Pharmaceutical Sciences, Faculty of Science, Utrecht University, TB Utrecht, The Netherlands |
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| Abstract: | The ‘sulfo‐click’ reaction, which is a chemoselective amidation reaction involving the reaction of an aminoethane sulfonyl azide with a thio acid, encompasses a new approach for ligation and conjugation. Detailed protocols are provided for decorating biologically active peptides or dendrimers with biophysical tags, fluorescent probes, metal chelators, and small peptides by using this reaction as a novel, metal‐free ‘sulfo‐click’ approach. Copyright © 2009 European Peptide Society and John Wiley & Sons, Ltd. |
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| Keywords: | amidation reactions bioconjugation chemoselectivity ‘ sulfo‐click’ thio acids/sulfonyl azides |
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