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A simple and efficient frit preparation method for one‐end tapered‐fused silica‐packed capillary columns in nano‐LC‐ESI MS |
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| Authors: | Feng Tan Shuo Chen Yangjun Zhang Yun Cai Xiaohong Qian |
| Institution: | 1. Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, School of Environmental Science & Technology, Dalian University of Technology, Dalian, P. R. China;2. State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, P. R. China |
| Abstract: | A novel frit preparation method for one‐end tapered‐fused silica‐packed capillary columns in nano‐LC‐ESI MS was developed. A hollow‐fused silica capillary column with a tapered tip as nano‐spray emitter was filled with 5 μm C18 beads, and then a sintered frit about 0.25 mm in length was prepared at the tip by butane flame. A stainless steel protection tube with 0.5 mm id was used to control the length of the frit and to protect the packed C18 beads behind the sintered frit during the sintering. C18 sintered frits were evaluated by BSA tryptic digests with nano‐LC‐LTQ. The sintered frits did not produce post‐column band broadening due to very small volume (about 0.2 nL) and did not produce adsorption to sample. The sintered frit columns had good separation reproducibility and separation performance compared with self‐assembled particles frit columns and commercial columns. |
| Keywords: | Frit preparation Nano‐LC‐ESI‐MS Packed capillary columns Technology |