Freeze-preservation of rice cells: A physiological study of freeze-thawed cells |
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| Authors: | Rino Cella Roberta Colombo Maria Grazia Galli Erik Nielsen Franco Rollo Francesco Sala |
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| Affiliation: | 1st. di Genetica Biochimica ed Evoluzionistica del C.N.R., Via S. Epifanio, 14, 27100 Pavia, Italy;1st. di Scienze Botaniche, Univ. de Milano, Italy;1st. di Microbiologia e Fisiologia vegetale, Univ. de Pavia, Italy. |
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| Abstract: | Rice ( Oryza sativa L.) cells returning to in vitro culture after preservation at superlow temperature in liquid nitrogen are characterized by a number of physiological alterations. These include: reduction in respiration and glucose uptake, loss of intracellular potassium, decrease in the cellular level of key metabolites (ATP, glucose-6-phosphate and pyruvate) and fragility of protoplasts following the action of cell wall-degrading enzymes.
Nevertheless, cell growth resumes after a short lag phase (2–4 days) with an actual 70–100% cell survival, thus indicating that the observed damage is not lethal and can be repaired in a short time. |
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| Keywords: | Cryoinjury cryopreservation rice |
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