TM4SF1在成纤维细胞与脐带间充质干细胞表达差异的研究

目的寻找成纤维细胞与脐带间充质干细胞（UCMSC）在细胞表面蛋白与分化能力方面的差异。 方法流式细胞术分析细胞表达CD105、CD90、CD73、CD44、CD14、CD34、CD45、CD79a、HLA-DR、FSP-1及TM4SF1的表达情况；细胞分化实验：观察成纤维细胞与UCMSC的成脂、成软骨及成骨能力；RT-PCR检测二者FSP-1、TM4SF1表达情况。两种细胞表型比较采用独立样本t检验。 结果成纤维细胞与UCMSC的表面分子CD105、CD90、CD73、CD44、CD14、CD34、CD45、CD79a、HLA-DR表达相似（P均> 0.05），都具有成脂、成软骨、成骨的分化能力；成纤维细胞与UCMSC FSP-1阳性细胞比例分别为（98.6±0.3323）﹪及（98.90±0.2665）﹪（t = 0.4677，P = 0.5294）；UCMSC TM4SF1阳性细胞比例为（97.23±0.2250）﹪，成纤维细胞TM4SF1阳性细胞比例为（0.0082±0.0018）﹪（t = 346.9，P < 0.01）。 结论TM4SF1在成纤维细胞与UCMSC上的表达量存在差异。

Difference of TM4SF1 expression between fibroblasts and UCMSC

ObjectiveTo find the difference between fibroblasts and umbilical cord mesemchemal stem cells (UCMSC) in surface markers and differentiation. MethodsFlow cytometric analysis of CD105, CD90, CD73, CD44, CD14, CD34, CD45, CD79a, HLA-DR, FSP-1 and TM4SF1 was performed for fibroblasts and UCMSC. Multilineage differentiation of fibroblasts and UCMSC into fat cells, cartilage cells and bone cells were induced. The expression of FSP-1 and TM4SF1 in fibroblasts and in UCMSC was assayed by RT-PCR. Data analysis between different groups was perfermed with independent-sample t test. ResultsThose markers which were found to be expressed on UCMSC were also expressed on fibroblasts in comparable levels (P > 0.05). Fibroblasts and UCMSC could differentiate into fat cells, cartilage cells and bone cells in vitro. Both fibroblasts and UCMSC were positive for FSP-1 (98.6±0.3323)﹪ vs (98.90±0.2665)﹪, (t?=?0.4677, P?=?0.5294). TM4SF1 was positive on UCMSC (97.23±0.2250)﹪, but negative on fibroblast (0.0082±0.0018)﹪(t?=?346.9, P?