| 采用RACE技术获得全长人新基因MAGE-D1 |
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| 引用本文： | 邢桂春,张成岗,魏汉东,贺福初. 采用RACE技术获得全长人新基因MAGE-D1[J]. 中国生物化学与分子生物学报, 2001, 17(2): 203-208 |
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| 作者姓名： | 邢桂春 张成岗 魏汉东 贺福初 |
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| 作者单位： | 军事医学科学院放射医学研究所 |
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| 基金项目： | 国家"863”项目(No.102-10-04-04)、国家自然科学基金重点项目(No.39730310,39970247) |
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| 摘 要： | c DNA末端快速扩增 (RACE)技术是快速获得新基因 5′和 3′端未知序列的有效手段 .鉴于新报导的人肿瘤基因 MAGE家族成员之一 MAGE- D1的 c DNA序列不完全 ,从而拟用 RACE技术获得 MAGE- D1的全长 c DNA序列 .结果显示 ,MAGE- D1的 c DNA全长为 2 80 0个碱基 ,编码778个氨基酸 .同时对 RACE技术应用时的一些关键问题进行了讨论 .
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| 关 键 词： | cDNA末端快速扩增技术 人黑色素瘤抗原相关基因D1 全长cDNA序列 |
| 收稿时间： | 2001-04-20 |
| 修稿时间： | 2000-07-17 |
Full-length Cloning of Human Melanoma Antigen-encoding Gene D1 Using Rapid Amplification of cDNA Ends |
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| XING Gui chun,ZHANG Cheng gang,WEI Han dong,HE Fu chu. Full-length Cloning of Human Melanoma Antigen-encoding Gene D1 Using Rapid Amplification of cDNA Ends[J]. Chinese Journal of Biochemistry and Molecular Biology, 2001, 17(2): 203-208 |
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| Authors: | XING Gui chun ZHANG Cheng gang WEI Han dong HE Fu chu |
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| Affiliation: | (Institute of Radiation Medicine,Academy of Military Medical Science,Beijing 100850,China |
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| Abstract: | Rapid amplification of cDNA ends(RACE)is a powerful technique to obtain unknown sequence of 5′ terminal or 3′ terminal part of a new gene.As the cDNA sequence of the newly discovered tumor related gene MAGE D1 is not in full length,RACE was used to study the 5′ terminal of this cDNA.The result showed that the full length cDNA sequence of MAGE D1 is 2800 bp in size and encodes a polypeptide of 778 amino acids.Some key problems were discussed in RACE. |
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| Keywords: | rapid amplification of cDNA ends human melanoma antigen encoding gene D1 full lenth cDNA sequence |
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