Direct detection of lactococcal bacteriophages in cheese whey using DNA probes |
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Authors: | Sylvain Moineau,José e Fortier,Sithian Pandian |
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Affiliation: | Centre de recherche STELA, Facultédes Sciences de l' Agriculture et de l' Alimentation, UniversitéLaval, Québec, Canada |
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Abstract: | Abstract We have designed a new method for the rapid detection of lactococcal phage directly in milk samples. The method was based on a dot blot analysis and did not require a biological assay with sensitive indicator strains. Culture media or whey permeate samples containing phage were spotted directly onto a nylon membrane and the phage were lysed in situ prior to hybridization. For skim milk, whole milk and whey samples, the samples were first treated with 50 mM EDTA, 20% Triton X-100 and heated at 60°C for 5 min, prior to spotting on the membrane. The detection limit was approximately between 105 and 107 pfu/spot. A large number of samples could be processed simultaneously and the results were obtained within 24 h. |
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Keywords: | Lactococcus Phage Probe Cheese Whey |
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