A liquid chromatography/electrospray ionization mass spectrometry (LC-MS/MS) assay for the determination of irinotecan (CPT-11) and its two major metabolites in human liver microsomal incubations and human plasma samples |
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| Authors: | D'Esposito Fabrizio Tattam Bruce N Ramzan Iqbal Murray Michael |
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| Affiliation: | Pharmacogenomics and Drug Development Group, Faculty of Pharmacy, The University of Sydney, NSW 2006, Australia. fabrizio@pharm.usyd.edu.au |
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| Abstract: | A sensitive, rapid LC-MS/MS assay has been developed and validated for the simultaneous quantification of CPT-11 and its two principal metabolites, 7-ethyl-10-hydroxycamptothecin (SN-38), and 7-ethyl-10-[4-N-(5-aminopentanoic acid)-1-piperidino]carbonyloxy-camptothecin (APC) in human liver microsomal fractions and plasma. The method was linear over the ranges of 1.56-100 ng/mL, 3.13-150 ng/mL, and 0.78-100 ng/mL for CPT-11, SN-38, and APC, respectively. The total run time was 7.0 min. This assay offers advantages in terms of expediency, recovery of analytes, and suitability for the analysis of CPT-11 and its metabolites in various biological fluids. |
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