Isolation of active 30S and 50S ribosomal subunits from a psychrotrophic bacterium |
| |
Authors: | F. A. Bertoli W. E. Inniss |
| |
Affiliation: | (1) Department of Biology, University of Waterloo, N2L 3G1 Waterloo, Ontario, Canada |
| |
Abstract: | Ribosomes from the psychrotroph,Bacillus insolitus, were successfully dissociated into 30S and 50S ribosomal subunits, which were active in carrying out in vitro protein synthesis, measured by the poly U-directed incorporation of14C-l-phenylalanine into polyphenylalanine. As opposed to the undissociated ribosomes, which are heat sensitive (30°C), the dissociated ribosomes were not thermally sensitive. The heat-sensitive component(s) was found to be removed from the ribosomes during dissociation into 30S and 50S ribosomal subunits; when added back to the ribosomal subunits, heat sensitivity was conferred. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|