Isolation of active 30S and 50S ribosomal subunits from a psychrotrophic bacterium |
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Authors: | F A Bertoli W E Inniss |
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Institution: | (1) Department of Biology, University of Waterloo, N2L 3G1 Waterloo, Ontario, Canada |
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Abstract: | Ribosomes from the psychrotroph,Bacillus insolitus, were successfully dissociated into 30S and 50S ribosomal subunits, which were active in carrying out in vitro protein synthesis,
measured by the poly U-directed incorporation of14C-l-phenylalanine into polyphenylalanine. As opposed to the undissociated ribosomes, which are heat sensitive (30°C), the dissociated
ribosomes were not thermally sensitive. The heat-sensitive component(s) was found to be removed from the ribosomes during
dissociation into 30S and 50S ribosomal subunits; when added back to the ribosomal subunits, heat sensitivity was conferred. |
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