Cloning, Sequencing, and Function of sanF: A Gene Involved in Nikkomycin Biosynthesis of Streptomyces ansochromogenes |
| |
Authors: | Wei Chen Hongmei Zeng Huarong Tan |
| |
Institution: | (1) Institute of Microbiology, Chinese Academy of Sciences, P.O. Box 2714, Beijing 100080, P. R. China, CN |
| |
Abstract: | A 111-bp DNA fragment related to nikkomycin biosynthesis of Streptomyces ansochromogenes 7100 was obtained with the method of reverse genetics. Then, a 2.2-kb DNA fragment was cloned from the DNA library of S. ansochromogenes 7100 by using the 111-bp fragment as a probe. Sequence analysis showed that the fragment contains one complete open reading
frame (ORF) that encodes a 219-amino acid (aa) protein, and this gene was designated sanF (GenBank Accession No. AF223971). The function of the sanF gene was studied by a strategy of gene disruption, and the resulting sanF mutants lost the ability to synthesize biologically active nikkomycin, indicating that sanF is essential for nikkomycin biosynthesis.
Received: 17 April 2000 / Accepted: 23 May 2000 |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|