再生丝素固定的酶标免疫传感器的研究

所研究的酶标免疫传感器是采用再生丝素将待测抗原 (兔IgG)固定在石墨电极表面 ,选用抗体 (山羊抗兔IgG HRP)与其识别结合。利用H₂O₂ 将抗原抗体结合的电位响应信号放大采用直接电位法检测IgG的浓度。该传感器测定IgG的最低浓度可达 1.2×10^-10 mol/L ,标准曲线的线形范围在4.1×10^-7～1.2×10^-10 mol/L ,回归方程为: E=-1049+721gIgG],响应时间为 15s。通过电泳的方法加速抗原抗体的识别结合 ,反应时间由原来的 90min缩短到 3 0min。这种以固定化抗原结合酶标抗体量的多少作为检测抗原标准的新型酶标免疫传感器 ,在临床检测、环境监测、HLA个人身份鉴定等领域都有着广阔的应用前景。

Study on Enzyme Immunosensor Immobilized by Regenerated Silk Fibroin

The author studies the enzyme immunosensor made up of graphite electrode and protein film of regenerated silk fibroin which is employed to immobilized antigen (rabbit IgG). IgG will be recognized and combined by antibody (goat-anti-rabbit IgG-HRP). After enlarging the signal of the combination of antigen and antibody by H2O2, the concentration of IgG is able to be measured by testing the electrode potential directly. The Enzyme Immunosensor is sensitive when measuring IgG and the detection limit of 1.2 x 10(-10) mol/L is found. It shows linear response over the concentration range of 4.1 x 10(-7)-1.2 x 10(-10) mol/L. Electrophoresis cuts down the time of antibody-antigen interaction from 90 min to 30 min. The response time is 15 s. Enzyme immunosensor with better stability and higher sensitivity can be used extensively in clinical diagnosis, medical and environmental studies, HLA molecular diagnosis and so on.