Characteristics of the interspecific somatic hybrids Solanum pinnatisectum (+) S. tuberosum H-8105 |
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Authors: | Anna Szczerbakowa Danuta Bołtowicz Renata Lebecka Paweł Radomski Bernard Wielgat |
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Affiliation: | (1) Institute of Biochemistry and Biophysics PAS, 5A Pawi skiego St., 02-106 Warsaw, Poland;(2) Plant Breeding and Acclimatization Institute (IHAR), Młochów Unit, 19 Platanowa St., 05-831 Młochów, Poland |
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Abstract: | The morphological, cytological and molecular analyses of the plants regenerated after PEG-induced fusion between mesophyll protoplasts from the dihaploid potato clone H-8105 and the wild tuberous disease-resistant species S. pinnatisectum, were performed. A single fusion experiment yielded 313 calli, although only two calli produced shoots. From the rooted shoots, two stable clones (PT-01-1 and PT-01-2) exhibiting different vigor and habitat, were developed. The plants of PT-01-1 clone grew slowly in vitro, produced tubers after transfer to soil but did not set flowers. In contrast, the plants of the vigorous clone PT-01-2 produced both tubers and flowers after transfer to soil. The flower and tuber morphology of PT-01-1 and PT-01-2 regenerants was intermediate in comparison to the parental species. Cytological analysis revealed that the PT-01-1 clone was pentaploid and the PT-01-2 clone was tetraploid. The molecular (RAPD) analysis confirmed hybridity of both clones. The preliminary tests on late blight resistance of the hybrids showed no differences with a potato parent. |
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Keywords: | potato Solanum pinnatisectum somatic hybridization ploidy RAPD late blight resistance |
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