| 应用噬菌体随机肽库技术筛选丙肝病毒NS3抗原模拟表位 |
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| 引用本文： | 钟彦伟,成军,等.应用噬菌体随机肽库技术筛选丙肝病毒NS3抗原模拟表位[J].Virologica Sinica,2002,17(3):202-205. |
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| 作者姓名： | 钟彦伟 成军 |
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| 基金项目： | 国家自然科学基金资助项目 (3 990 0 13 0 ) |
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| 摘 要： | 以抗 HCVNS3的单克隆抗体作为固相筛选分子 ,对人工合成的噬菌体随机 12肽库进行 5轮“吸附 洗脱 扩增”的筛选过程 ,随机挑取 4 2个克隆 ,经噬菌体酶联免疫吸附法 (ELISA)鉴定并进行交叉反应实验以及竞争抑制性结合实验 ,最后对所选克隆进行DNA序列分析 ,以确定HCVNS3抗原的模拟表位。经噬菌体富集后 ,从随机筛选的 4 2个克隆中得到 11个阳性克隆 ,确定氨基酸序列XXIXXXXMSNXX为HCVNS3的模拟表位。我们用噬菌体12肽库成功筛选得到HCVNS3的模拟表位 ,为开展用HCV模拟表位探索HCV的防治研究创造了条件
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| 关 键 词： | 丙型肝炎病毒 非结构蛋白NS3 模拟表位 噬菌体肽库 |
Epitope Mapping of Hepatitis C Virus NS3 Antigen from a Phage Peptide Library by Using Immobilized Specific Monoclonal Antibody |
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| ZHONG Yan wei,CHENG Jun,CHEN Xin hua,WANG Gang,HONG Yuan,WANG Lin,LI Li,ZHANG Ling xia,CHEN Ju mei.Epitope Mapping of Hepatitis C Virus NS3 Antigen from a Phage Peptide Library by Using Immobilized Specific Monoclonal Antibody[J].中国病毒学(英文版),2002,17(3):202-205. |
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| Authors: | ZHONG Yan wei CHENG Jun CHEN Xin hua WANG Gang HONG Yuan WANG Lin LI Li ZHANG Ling xia CHEN Ju mei |
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| Abstract: | Using HCV NS3 monoclonal antibody as selective molecule, a 12 mer phage peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing. Eleven positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was confirmed as mimotope of HCV NS3. HCV NS3 mimotope was obtained by phage peptide library screening. The result provides a new approach for HCV therapy and vaccine development. |
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| Keywords: | Hepatitis C virus NS3 Mimotopes Phage peptide library |
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