Radioimmunoassay of plasma androsterone |
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| Authors: | E Youssefnejadian W P Collins I F Sommerville |
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| Affiliation: | Department of Biochemical Endocrinology, Chelsea Hospital for Women, Dovehouse Street, London, S.W.3. England |
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| Abstract: | A method is described and evaluated for the determination of androsterone in peripheral venous plasma (1.0 ml) from men and women. The procedure involves addition of labelled internal standard and extraction with diethyl ether. Aliquots (10 %) are removed for radioimmunoassay. An antiserum to androsterone-17-carboxymethyl oxime-bovine serum albumin and tritiated androsterone complete the system. The practical systematic errors have been determined by replicate analyses. The range of values (mean ± S.D.) in plasma from 40 healthy men are 54 ± 32 ng/100 ml, and the corresponding values for women 46 ± 28. |
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