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Genetic characterisation of uninucleated cyst-producing Entamoeba spp. from ruminants |
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| Authors: | C Rune Stensvold Marianne Lebbad |
| Institution: | a Laboratory of Parasitology, Statens Serum Institut, Copenhagen, Denmark b Department of Parasitology, Mycology and Environmental Microbiology, Swedish Institute for Infectious Disease Control, Solna, Sweden c Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom |
| Abstract: | Six ssrRNA gene sequences were obtained by PCR amplification of DNA from uninucleated Entamoeba cysts isolated from fresh faeces of sheep, cows, a roe deer and a reindeer. Phylogenetic analysis using sequences of non-, uni-, quadri- and octonucleate cyst-producing Entamoeba spp. for comparison showed that all six isolates formed a separate clade nested within the clade of quadrinucleate cyst producers. The data indicate that Entamoeba bovis can be isolated from ruminant hosts other than cattle, and we suggest that organisms clustering with the sheep and cattle isolates analysed in the present study be named E. bovis. |
| Keywords: | Entamoeba Parasite Ruminants Genetic diversity PCR |
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