Ribosomal DNA Sequence of Nucleospora salmonis Hedrick, Groff and Baxa, 1991 (Microsporea: Enterocytozoonidae): Implications for Phylogeny and Nomenclature |
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| Authors: | MARGARET F DOCKER MICHAEL L KENT DOMINIQUE M L HERVIO JASWINDER S KHATTRA LOUIS M WEISS ANN CALI ROBERT H DEVLIN |
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| Institution: | West Vancouver Laboratory, Fisheries and Oceans Canada, 4160 Marine Drive, West Vancouver, British Columbia V7V 1N6, Canada.;Pacific Biological Station, Fisheries and Ocens Canada, Nanaimo, British Columbia V9R 5K6, Canada.;Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA;Department of Biological Sciences, Rutgers University, Newark, New Jersey 07102, USA |
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| Abstract: | ABSTRACT. Rules of zoological nomenclature, morphological data, and robosomal DNA sequence data support the validity of the genus Nucleospora , and its placement in the family Enterocytozoonidae. Although Nucleospora exhibits most of the distinguishing morophological characteristics of the family Enterocytozoonidae Cali and Owne, 1990, the distinctively different hosts (fish and humans, respectively) and sites of development (the nuclei of immature blood cells and the cytoplasm of enterocutes) supprot the placement Nucleospora and Enterocytozoon into separate genera. Ribosomal DNA sequence comparisons between Nucleospora salmonis and Enterocytozoon bieneusi showed 19.8% genetic divergece in the large and small subunit regions. Although more inter- and intrageneric divergence between the two species is sufficiently larg to deter suppression of the genus Nucleospora as a junior synonym of Enterocytozoon. A polymerase chain reaction test for the detection of N. salmonis in chinook salmon ( Oncorhynchus tshawytscha ), based on N. salmonis -specific ribosomal DNA sequence, is described. |
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| Keywords: | Chinook salmom diagnosis Enterocytozoon salmonis parasite polymerase chain reaction |
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