Phosphorylation-dependent phospholipase D activity of matrix vesicles |
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Authors: | Balcerzak Marcin Pikula Slawomir Buchet Rene |
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Affiliation: | Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093 Warsaw, Poland. |
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Abstract: | A progressive hydrolysis of phospholipids was observed during the mineralization process mediated by extracellular matrix vesicles. Increasing levels of different hydrolysis products revealed phospholipase A and D activities. The importance of these enzymes for the mineralization process lies in a high rate of hydrolysis of neutral phospholipids and lower rate of degradation of anionic phospholipids, which may favor mineral formation in vesicular membrane and membrane breakdown necessary for the release of mineral deposits into extracellular matrix. In this report, we focus on the phosphorylation-dependent phospholipase D activity during mineral formation initiated by chicken embryo matrix vesicles. |
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Keywords: | Matrix vesicles Mineralization Phospholipase C Phospholipase D Phosphorylation |
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