Cloning of DNA sequences from Methanococcus vannielii capable of autonomous replication in yeast |
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| Authors: | Leo Meile Thomas Leisinger John N Reeve |
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| Institution: | (1) Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule Zürich, CH-8092 Zürich, Switzerland;(2) Department of Microbiology, The Ohio State University, 43210 Columbus, OH, USA |
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| Abstract: | Total DNA of the archaebacterium Methanococcus vannielii was digested with BamHI or BamHI/HindIII, cloned with plasmid Yip5 and analyzed for sequences capable of autonomous replication (ARSs) in the eukaryote Saccharomyces cerevisiae. Two recombinant plasmids were isolated which contained 3.3 kb and 8 kb fragments of methanogen derived DNA with ARS activity. They exhibited low transformation efficiencies for yeast and promoted slow growth of yeast transformants.Abbreviations Ap
ampicillin
- ARS
autonomously replicating sequence
- EtBr
ethidium bromide
- kb
kilobase(s)
- Mc.
Methanococcus
- R
resistance
- RE
replication enhancer
- RS
replication sequence
- Tc
tetracycline |
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| Keywords: | Autonomously replicating sequence (ARS) Methanococcus vannielii Archaebacteria Replication origin |
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