Preparation and characterization of new challenge stocks of SIVmac32H J5 following rapid serial passage of virus in vivo |
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Authors: | Ferguson D Wade-Evans A Elsley W Sangster R Silvera P MacManus S Davis G Corcoran T Berry N Brown S Jenkins A Cowie J Sethi M Hull R Stebbings R Lines J Norley S Stott E J Almond N |
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Affiliation: | Division of Retrovirology, NIBSC, South Mimms, Potters Bar, Hertfordshire, UK. dferguson@nibsc.ac.uk |
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Abstract: | BACKGROUND: A new challenge stock of the simian immunodeficiency virus SIVmacJ5 has been produced following passage in vivo. METHODS: SIVmacJ5 3/92 (J5M), was passaged serially through cynomolgus macaques (Macaca fascicularis) by intravenous inoculation of infected spleen cells isolated and prepared 14 days post-infection. Two challenge stocks, SIVmacJ5 S61MLN and SIVmacJ5 S62spl, were prepared by culture of lymphoid tissue ex vivo. RESULTS: These virus stocks appeared better adapted for replication in M. fascicularis as demonstrated by a greater persistence of recoverable live virus from the periphery and increased pathology in lymphoid tissues 20 weeks post-challenge as detected by immunohistochemistry. Sequence analysis of the envelope gene from these stocks did not identify marked diversification of sequence as a result of this procedure. CONCLUSIONS: These stocks display more robust peripheral persistence and tissue pathology in cynomolgus macaques and should prove valuable analysing recombinant vaccines based upon SIVmacJ5 transgenes. |
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Keywords: | Cynomolgus macaque immunohistochemistry in situ hybridization pathology SIVmacJ5 S61MLN |
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