Haem a, cytochrome c and total protein turnover in mitochondria from rat heart and liver |
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| Authors: | V. Aschenbrenner R. Druyan R. Albin M. Rabinowitz |
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| Affiliation: | Departments of Medicine and Biochemistry, The Pritzker School of Medicine, University of Chicago, Chicago, Ill. 60637, U.S.A., and the Argonne Cancer Research Hospital, Chicago, Ill. 60637, U.S.A. |
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| Abstract: | Haem a and cytochrome c were isotopically labelled in mitochondria from rat heart and liver after injection of delta-amino[2,3-(3)H(2)]laevulate, a specific haem precursor. [guanido-(14)C]Arginine or l-[4,5-(3)H(2)]leucine were used to label mitochondrial proteins. Half-lives were measured from biological decay in vivo and were similar (5.5-6.2 days) for haem a, cytochrome c and [(14)C]arginine-labelled proteins. Labelling of hepatic mitochondrial proteins with [(3)H(2)]leucine resulted in a prolonged apparent half-life. |
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