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| 抗哇巴因鸡蛋黄IgY与兔抗体IgG在酶联免疫检测中的比较 | |
| 作者姓名： | Zhang MJ Yang J Ge H Qiang L Duan ZM Wang CX Wang R Lu ZR |
| 作者单位： | 1. 西安交通大学医学院第二附属医院心内科,陕西,西安,710004 2. 西安交通大学医学院第二附属医院病理科,陕西,西安,710004 3. 西安交通大学医学院第一附属医院干部病房,陕西,西安,710061 |
| 基金项目： | 陕西省社发攻关项目资助（2005K11-G3（1）） |
| 摘 要： | 目的：探讨提高内源性哇巴因（EO）检测方法的准确性及特异性,为哇巴因的研究打下基础。方法：分别制备出鸡蛋黄抗哇巴因多克隆抗体及兔抗体。然后采用酶联免疫吸附法（ELISA）比较两种抗体检测内源性哇巴因的准确性及特异性。结果：采用IgY检测EO的平均批内变异系数为2．03％,批间变异系数为2．34％。兔抗体IgG则分别为2．83％、3．29％;两者均与氢化可的松及地塞米松无交叉结合反应,与西地兰和地高辛分别存在3．45％vs5．95％、3．20％vs5．20％的交叉反应。结论：IgY比兔抗体ELISA检测内源性哇巴因的特异性及准确性较高。 |
| 关 键 词： | 抗哇巴因 |
| 文章编号： | 1000-6834（2007）04-0505-04 |
| 收稿时间： | 2006-02-14 |
| 修稿时间： | 2007-03-20 |
Comparison between anti-ouabain egg yolk(IgY) and rabbit antibody(IgG) in enzyme-linked immunosorbent assay |
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| Zhang MJ,Yang J,Ge H,Qiang L,Duan ZM,Wang CX,Wang R,Lu ZR.Comparison between anti-ouabain egg yolk(IgY) and rabbit antibody(IgG) in enzyme-linked immunosorbent assay[J].Chinese Journal of Applied Physiology,2007,23(4):505-508. | |
| Authors: | Zhang Ming-juan Yang Jun Ge Heng Qiang Lei Duan Zong-ming Wang Cong-xia Wang Rong Lu Zhuo-rern |
| Institution: | Department of Cardiology, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China. |
| Abstract: | Aim:To improve specificity and accuracy of endogenous ouabain measurement assay.Methods:Anti-ouabain polyclonal antibody egg yolk(IgY) and anti-ouabain rabbit antibody(IgG) were prepared respectively.In the presence of two kinds of antibody,then the specificity and accuracy of enzyme-linked immunosorbent assay(ELISA) were compared.Results:The ELISA,in the presence of IgY,provided a sensitivity of the average intraassay coefficient of variation(CV) was 2.03%,and the inter-assay CV was 2.34% respectively.In contrast,IgG were 2.83% and 3.29%.No significant interferences were observed with hydrocortisone and dexamethasone.There was 3.45% vs 5.95%,3.20% vs 5.20% of crossreaction with cedilanid and digoxin.Conclusion:The specificity and accuracy of ELISA,in which IgY was used,were more better than IgG. |
| Keywords: | IgY IgG |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |